PRODUCTS – IM-T(T-lymphocyte culture medium)PRODUCTS

Comparison of the performance of a medium containing serum, which is currently used at our company, with “iMediam for T” (Day 14 of culture)

Surface antigen analysis

Peripheral blood mononuclear cells (PBMC) from 3 healthy individuals who gave informed consent were cultured using “iMediam for T” containing no serum for 14 days. Then, the ratio of CD3-positive cells, which is a T-lymphocyte index, was measured by a flow cytometer. The ratio of CD3 was 98% or above in all 3 specimens, which indicates that T-lymphocytes were selectively propagated.

Specifications

• Application: Human T-lymphocyte.
• Storage condition: Cold dark place (2–10ºC).
• Sterility tests: ○ Endotoxin: Colorimetric method ○ Mycoplasma: Culture method ○ Fungus and bacteria: Agar plate surface-spread method
• Procedure: (1) Prepare OKT3 antibody-immobilized flask 225. (2) Suspend 3–5 × 10⁷ cells of PBMC into 30–50 mL of “iMediam for T”. (3) Add an appropriate amount of “iMediam for T” depending on the condition of cell proliferation. (4) After 5–7 days of culture, transfer the cell suspension to gas-permeable culture bags, add an appropriate amount of “iMediam for T”, and continue to culture for another 2–3 weeks. Increase the number of culture bags depending on the culture condition.
• Notes: ○ Do not use on the human body. ○ We shall not be held responsible for accidents or damage resulting from using this product. ○ If you have any questions about the usage, please contact GC LYMPHOTEC, Inc.

• Product code

  iM-T

• Product name

  iMediam for T

• Use

  Human T-lymphocyte culture

• Volume

  1,000mL