BAMBANKER is a serum-free medium for long-term freezing at -80℃ and preservation of valuable culture cells such as tumor and normal cells.
＜For Research Use Only＞
Features
- 1) Serum-free
- 2) Ready-to-use medium for preservation of cells
- 3) No program freezer is required
- 4) Rapid and long-term freezing and preservation in a deep freezer
- 5) Usable for 2years at 2～4℃
Operating Procedure
- 1) Centrifuge the cells in logarithmic growth phase and collect 5×105 ～ 1×107cells.
- 2) Suspend the collected cells in 1mL of this medium and place the cells in cryotubes for freezing and preservation.
Then freeze and preserve the cells at -80℃ without preliminary freezing. The cells frozen at -80℃ can be preserved
subsequently in liquid nitrogen. - 3) Thawing must be carried out quickly in a constant-temperature chamber or bath.
- ★ The cells must be frozen in logarithmic growth phase.
Application（Cell preserved well by cryopreservation test)
- ・P3U1 (mouse myeloma cell line) ・K562(human leukemia cell line) ・Human gastric epithelial cells
・humanγδT cell ・Daudi (human B cell line) ・PC12 (rat-derived adrenal phechromocytona)
・human B cell line ・OKT4 (mouse hybridoma) ・monkey Bcell line ・Mouse ES cell line
・Activated lymphocyte derived from human peripheral blood ・Activated lymphocyte derived from mouse spleen
