PRODUCTS – BAMBANKER®PRODUCTS

The following data was provided by Dr. Atsunori Sato of Tagawa Laboratory, School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology.

Customer Evaluation from Mr. Shota Moriya, Department of Biochemistry, Tokyo Medical University, is available.

Comparative video of preservation efficiency of mouse fibroblast cell line

Bambanker®

Self-prepared preservative solution (FBS containing 10% DMSO)

Experimental conditions • Cells used: Mouse fibroblast cell lines (MEF obtained from American Type Culture Collection) • Number of cells stored: 4×106 cells/vial. Each cell was suspended in 1 ml of preservative solution. • Storage temperature: -80ºC. Cells were directly placed in a storage box and cooled without using BICELL, etc. • Storage period: 45 days

A number of dead cells as well as stagnation of growth potential were observed in self-prepared preservative solution; however, Bambanker® showed vigorous cell proliferation ability immediately after thawing, and survival of almost all cells was confirmed. (For details, refer to the user’s report at the top of the page)

BAMBANKER® is a serum-free medium for long-term freezing at -80℃ and preservation of valuable culture cells such as tumor and normal cells.

＜For Research Use Only＞

Features

1. Serum-free
2. Ready-to-use medium for preservation of cells
3. No program freezer is required
4. Rapid and long-term freezing and preservation in a deep freezer
5. zUsable for 2years at 2～4℃

Operating Procedure

1. Centrifuge the cells in logarithmic growth phase and collect 5×105 ～ 1×107cells.
2. Suspend the collected cells in 1mL of this medium and place the cells in cryotubes for freezing and preservation. Then freeze and preserve the cells at -80℃ without preliminary freezing. The cells frozen at -80℃ can be preserved subsequently in liquid nitrogen.
3. Thawing must be carried out quickly in a constant-temperature chamber or bath.

★ The cells must be frozen in logarithmic growth phase.