iStock 

Features of iStock 

  • Product for regenerative medicine research
  • Good for ES and iPS cells research 
  • Ready-to-use preservation medium can be used without diluting
  • Programmable freezer not required
  • Rapid and long-term freezing and preservation of frozen cells at −80°C
  • Contains no serum or substances derived from other organisms
  • Xeno-free , Serum-free

Storage: Cool, dark place (at 2–10°C) Shelf life: 3 year from the date of manufacture

This product has approval from PMDA  as raw materials can be used for regenerative medicine products.


Conditions

Cells used: mouse ES cell

Freezing conditions:
Proliferated mouse ES cells were adjusted to a concentration of 4 × 106 cells / mL using 10% DMSO / Medium, iStock, product A, and product B.
1ml of the suspension was dispensed into a cryopreservation tube (n = 4)

Thawing conditions:
The cells were thawed rapidly at 37℃, centrifuged and were seeded with culture vessel.

Test after thawing:
The viable cell number was measured by Trypan Blue exclusion staining.

Results:
iStock showed a higher recovery rate than other medium.


CAUTION

  • Use cells during the logarithmic growth phase
  • Cannot be used for the human body
  • Do not use for cell preservation aiming at treatment
  • Do not use for any purpose other than research
  • Before using this product, conduct a confirmation test using the desired cells
  • We shall not be responsible for accidents or damage resulting from the use of this product

Distributor

FUJIFILM Wako Pure Chemical Corporation

https://www.fujifilm.com/ffwk/en